Tissue Culture and Plant Regeneration

Description

“The term ‘Tissue culture’ can be applied to any multicellular culture growing on a solid medium that consists of many cells in protoplasmic continuity. The culture of an explant, consisting of one or more tissue, results in a callus that has no structural or functional counterpart with any tissue of the normal plant body. Tissue culture, a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. Regeneration of plants from cultured cells has many other applications. Plant regeneration from cultured cells is proving to be a rich source of genetic variability, called “somaclonal variation”. Several somaclones have been processed into new cultivars. Regeneration of plants from microspore/pollen provides the most reliable and rapid method to produce haploids, which are extremely valuable in plant breeding and genetics. Plant regeneration is the major outcome of plant tissue culture, which is based on the principle of totipotency. Plant regeneration can be achieved by organogenesis and somatic embryogenesis. Organogenesis means formation of organs from the cultured explants. This book is aimed at all those interested and involved in the results of plants on the environment and is an essential basic reference book for students, academicians and specialists.